Nzomics
a blog about nitrile-active enzymes
Tuesday, 27 September 2016
Preparation of CLEAs of a recombinant NHase ES-NHT-118
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Preparation of Cross-linked Enzyme Aggregates of Nitrile Hydratase ES-NHT-118 from E. coli by Macromolecular Cross-linking Agent Liy...
Thursday, 1 September 2016
ChromSoc nitrilase flow chemistry project 5
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We have looked at the conversion of 4-cyanopyridine to isonicotinic acid using our mesoscale flow chemistry apparatus containing a nitrilas...
Friday, 5 August 2016
The cysteinesulfenic Acid in NHase as catalytic nucleophile
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Time-Resolved Crystallography of the Reaction Intermediate of Nitrile Hydratase: Revealing a Role for the Cysteinesulfenic Acid Ligand a...
High concentration synthesis of 3-hydroxypropionic acid
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Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli ...
Simultaneous KRED and NHase/amidase activity
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Developing a Biocascade Process: Concurrent Ketone Reduction-Nitrile Hydrolysis of 2-Oxocycloalkanecarbonitriles Elisa Liardo, Nicolás R...
Wednesday, 3 August 2016
ChromSoc nitrilase flow chemistry project 4
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Once you have the track filled with immobilized enzyme and the two halves stuck together, you just need to condition it and check there are ...
ChromSoc nitrilase flow chemistry project 3
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Rob has shown made a supply of the plates that go together to make a flow cell for flow biocatalysis. The 3D printed master copy (the one w...
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