NHases in organisms that aren't bacterial

A protein search amongst NCBI or SwissProt's data for NHases that arent in bacteria has been a pretty fruitless task. There are two notable exceptions to the "NHases are in bacteria, period" rule. The paper [Foerstner KU, Doerks T, Muller J, Raes J, Bork P. A nitrile hydratase in the eukaryote Monosiga brevicollis. PLoS ONE 2008;3:e3976.] showed that there is something in an aquatic metazoan Monsiga brevicollis which is combines the alpha and beta subunit in one protein chain. That sounded interesting so we have done some molecular biology magic and put that sequence in E. coli, and it expresses OK, though we havent had the time/manpower to play with it yet. The other outlier is pointed out in Prasad and Balla's Biotech Advances review of last year which points out that the plant Ricinus communis has also got the primary sequence for a NHasetoo. The usual tag sequence (VCTLCSC) in the alpha subunit suggests it is a cobalt centred one and if you ClustalW that with the cobalt-centred NHase which is featured in pdb file 1V29 (from Bacillus smithii) you get an alignment score of 54. Interesting.