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Friday, 12 August 2011

Proper NCBI numbers for August

I have been looking for a number for the actual number of proper NHase alpha subunit sequences on record. I have done this by really combing through the NCBI protein records using a BLASTp approach for similarity, and excluding sequences that aren't an appropriate length nor have the usual active site binding motif. I have ignored environmental samples, and dropped out data from PDB files.
As of the start of this week (8th Aug 2011) I reckon there are about 190 NHase alpha sequences. That breaks down as about 25% iron and 75% cobalt centred. There are four from eukaryotic organisms (three marine and one plant), and the rest are... not.

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