A new thermophilic
nitrilase from an Antarctic hyperthermophilic microorganism by Geraldine V. Dennett and Jenny M. Blamey
Several environmental samples from Antarctica
were collected and enriched to search for microorganisms with nitrilase
activity. A new thermostable nitrilase from a novel hyperthermophilic archaea Pyrococcus sp. M24D13 was purified and
characterized. The activity of this enzyme increased as the temperatures rise
from 70 up to 85 °C. Its optimal activity occurred at 85 °C and pH 7.5. This
new enzyme shows a remarkable resistance to thermal inactivation retaining more
than 50% of its activity even after 8 h of incubation at 85 °C. In addition,
this nitrilase is highly versatile demonstrating activity towards different
substrates such as benzonitrile (60 mM, aromatic nitrile) and
butyronitrile (60 mM, aliphatic nitrile). Moreover the enzyme NitM24D13 also
presents cyanidase activity.
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