E. coli expression of active nitrile hydratase from Aurantimonas manganoxydans needs cobalt ions

This paper in Biotechnology Letters from Pei and co-workers gives details of a cobalt centred nitrile hydratase from Aurantimonas manganoxydans, and the importance of getting the concentration right for good level of expression and activity. (DOI 10.1007/s10529-013-1215-5)