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Friday, 30 August 2013

Zaparucha nitrilase activities

One of the joys of the Zaparucha nitrilase paper recently published in Advanced Synthesis and Catalysis is that their search for nitrilase activity is not confined to those enzymes which have already been automated annotated as "nitrilase". Within their enzyme set are proteins annotated as amidases, cyanide hydrolases, ureidopropionases, hydrolases (nice and broad that!) and even glycosyl. Here is my analysis of their protein types done by text searching their supplementary materials.




Text search strings are across the top and obviously "nitrilase" will also find those in "nitrilase/cyanide", and "hydrolase" occurs all over the shop. The second line indicates the totals after my removal of those proteins which didnt show any nitrilase activity for them. (You can take the chemist stance "something wrong with the protein" or the molecular biologists stance "you havent found the right substrate yet" at your own whim!). A very interesting set of annotations!

 

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