Antonie van Leeuwenhoek has a paper (Expression control of nitrile hydratase and amidase genes in Rhodococcus erythropolis and substrate specificities of the enzymes) from Nesvera and
co-workers at the Institute of Microbiology in Prague which looks at the
expression of the aldoxime dehydratase/nitrile hydratase/amidase pathway in two
different strains of NHase from Rhodococcus
erythropolis- A4 and CCM2595. It also looks at some of the selectivity of
the constituent enzymes in this pathway towards cyanohydrins. They make some
comparisons to the AJ270 strain which we published on in 2011. Rather like
us, they find that Rhodococcus
erythropolis strains are not very enantioselective NHases for mandelonitrile
derivatives- our paper reports E=2 for unadorned mandelonitrile.
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