Journal of Industrial Microbiology & Biotechnology, DOI 10.1007/s10295-016-1747-5
This paper describes a simple and effective method to
immobilize recombinant nitrilase, for efficient production of
2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio)
butanenitrile. The immobilized enzyme displayed better thermal stability, pH
stability and shelf life compared to free nitrilase. Moreover, it showed
excellent reusability and could be recycled up to 16 batches without
significant loss in activity. 200 mM 2-hydroxy-4-(methylthio) butanenitrile was
completely converted by the immobilized enzyme within 30 min, and the
accumulation amount of 2-hydroxy-4-(methylthio) butanoic acid reached 130
mmol/g of immobilized beads after 16 batches.
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