When expression doesn't work...

In the light of my comment in the last blog post commending the Clarke review for its table of successfully expressed enzymes, I thought I would put up here the following information... two genome sourced sequences, which in E.coli, gave inactive protein.

 >gi|50084777|ref|YP_046287.1| along with its beta subunit from Acinetobacter sp. ADP1 which we predicted would be an iron centred NHase
>gi|27379609|ref|NP_771138.1| along with its beta subunit from Bradyrhizobium japonicum USDA 110 which you would predict from its sequence to be a cobalt centred NHase.

Not sure why these didn't work... but with the wealth of sequences out there, there is always another one to aim at!