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Thursday, 23 June 2011

A more extreme than usual NHase

There is a cobalt centred nitrile hydratase from Rhodococcus opacus which has been the subject of work by Yasukawa and patents and commercial usage by Degussa. If you BLASTp the alpha chain of this, as NCBI titles it, "unnamed protein product" against their RefSeq collection, you find there are a lot of similar NHases. One that is relatively new comes from the newly released draft genome sequence of the bacterium Caldalkalibacillus thermarum TA2.A1. There is a good clue in its name to the fact that this bacterium grows optimally 65-70 degrees C and pH 9.5. That is pretty hot for a NHase, and with those pH conditions, no surprise it isnt an iron-centred version!

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