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Wednesday 15 May 2013

What might happen after the nitrile hydratase has done its job in a whole cell system?

...You've got to work with the amidase which converts the amide to the corresponding carboxylic acid.
Laura Cantarella, Alberto Gallifuoco, Agata Spera and Maria Cantarella look at the amidase in the NHase/amidase system of Microbacterium imperiale CBS 498-74 in the paper entitled "Nitrile, amide and temperature effects on amidase-kinetics during acrylonitrile bioconversion by nitrile-hydratase/amidase in-situ cascade system" in the journal Bioresource Technology with DOI:10.1016/j.biortech.2013.04.126.

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