Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
Wednesday 15 May 2013
Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions
This is a paper in Journal of Biotechnology by Jie Chen, Huimin Yu, Changchun Liu, Jie Liu and Zhongyao Shen (doi:10.1016/j.jbiotec.2013.01.021) on methodology to stabilize notoriously temperature sensitive nitrile hydratase. It involves the transfer of three regions which are prone to thermal deformation from thermophilic Bacillus SC-105-1 and Pseudonocardia thermophila JCM3095 into mesophilic Rhodococcus ruber TH.
Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
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