Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
Showing posts with label thermal stabilization. Show all posts
Showing posts with label thermal stabilization. Show all posts
Wednesday, 15 May 2013
Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions
This is a paper in Journal of Biotechnology by Jie Chen, Huimin Yu, Changchun Liu, Jie Liu and Zhongyao Shen (doi:10.1016/j.jbiotec.2013.01.021) on methodology to stabilize notoriously temperature sensitive nitrile hydratase. It involves the transfer of three regions which are prone to thermal deformation from thermophilic Bacillus SC-105-1 and Pseudonocardia thermophila JCM3095 into mesophilic Rhodococcus ruber TH.
Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
Three types of salt bridges—active-center-adjacent (in A1), internal neighboring-residue-bridged (in A2) and C-terminal-residue-bridged (A3) were tried but only the type with A3 had increased efficacy at higher temperatures.
Subscribe to:
Posts (Atom)