In the light of my comment in the last blog post commending the Clarke review for its table of successfully expressed enzymes, I thought I would put up here the following information... two genome sourced sequences, which in E.coli, gave inactive protein.
>gi|50084777|ref|YP_046287.1| along with its beta subunit from Acinetobacter sp. ADP1 which we predicted would be an iron centred NHase
>gi|27379609|ref|NP_771138.1| along with its beta subunit from Bradyrhizobium japonicum USDA 110 which you would predict from its sequence to be a cobalt centred NHase.
Not sure why these didn't work... but with the wealth of sequences out there, there is always another one to aim at!
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I am told, we have got these working now. Not sure what the magic was- something to do with molecular biology, I suspect.
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